Understanding genome regulation requires capturing chromatin dynamics in real time. In a recent issue of Cell, Zhu et al. introduce Oligo-LiveFISH,1 a high-resolution live-cell imaging platform that tracks non-repetitive loci without genome engineering. By assembling fluorescent guide RNA pools with dCas9, this method enables 20-nm, 50-ms resolution imaging, offering insights into dynamic genome organization across cell lines and primary cells.
Visualizing chromatin communication in living cells through Oligo-LiveFISH / Conte, Mattia; Nicodemi, Mario. - In: CELL REPORTS. METHODS. - ISSN 2667-2375. - 5:6(2025). [10.1016/j.crmeth.2025.101079]
Visualizing chromatin communication in living cells through Oligo-LiveFISH
Conte, Mattia
Primo
;Nicodemi, Mario
Ultimo
2025
Abstract
Understanding genome regulation requires capturing chromatin dynamics in real time. In a recent issue of Cell, Zhu et al. introduce Oligo-LiveFISH,1 a high-resolution live-cell imaging platform that tracks non-repetitive loci without genome engineering. By assembling fluorescent guide RNA pools with dCas9, this method enables 20-nm, 50-ms resolution imaging, offering insights into dynamic genome organization across cell lines and primary cells.| File | Dimensione | Formato | |
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