STING is a transmembrane ER resident protein that was initially described as a regulator of innate immune response triggered by viral DNA and later found to be involved in a broader range of immune processes. Here, we assessed its role in the antigen presentation by generating a STING KO macrophage cell line. In the absence of STING, we observed an impaired OVA-derived SIINFEKL peptide presentation together with a decreased level of MHC-I complex on the plasma membrane, likely due to a decreased mRNA expression of β2 m light chain as no relevant alterations of the peptide-loading complex (TAPs) were found. Moreover, JAK-STAT signaling resulted in impaired STING KO cells following OVA and LPS treatments, suggesting a dampened activation of immune response. Our data revealed a new molecular role of STING in immune mechanisms that could elucidate its role in the pathogenesis of autoimmune disorders and cancer.

The Lack of STING Impairs the MHC-I Dependent Antigen Presentation and JAK/STAT Signaling in Murine Macrophages / Caiazza, Carmen; Brusco, Teresa; D'Alessio, Federica; D'Agostino, Massimo; Avagliano, Angelica; Arcucci, Alessandro; Concetta, Ambrosino; Giuseppe, Fiume; Mallardo, Massimo. - In: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES. - ISSN 1422-0067. - 23:22(2022), pp. 1-16. [10.3390/ijms232214232]

The Lack of STING Impairs the MHC-I Dependent Antigen Presentation and JAK/STAT Signaling in Murine Macrophages

Carmen Caiazza;Brusco Teresa;Federica D'Alessio;Massimo D'Agostino;Angelica Avagliano;Alessandro Arcucci;Massimo Mallardo
2022

Abstract

STING is a transmembrane ER resident protein that was initially described as a regulator of innate immune response triggered by viral DNA and later found to be involved in a broader range of immune processes. Here, we assessed its role in the antigen presentation by generating a STING KO macrophage cell line. In the absence of STING, we observed an impaired OVA-derived SIINFEKL peptide presentation together with a decreased level of MHC-I complex on the plasma membrane, likely due to a decreased mRNA expression of β2 m light chain as no relevant alterations of the peptide-loading complex (TAPs) were found. Moreover, JAK-STAT signaling resulted in impaired STING KO cells following OVA and LPS treatments, suggesting a dampened activation of immune response. Our data revealed a new molecular role of STING in immune mechanisms that could elucidate its role in the pathogenesis of autoimmune disorders and cancer.
2022
The Lack of STING Impairs the MHC-I Dependent Antigen Presentation and JAK/STAT Signaling in Murine Macrophages / Caiazza, Carmen; Brusco, Teresa; D'Alessio, Federica; D'Agostino, Massimo; Avagliano, Angelica; Arcucci, Alessandro; Concetta, Ambrosino; Giuseppe, Fiume; Mallardo, Massimo. - In: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES. - ISSN 1422-0067. - 23:22(2022), pp. 1-16. [10.3390/ijms232214232]
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Descrizione: 2022. International Journal of Molecular Sciences
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11588/906072
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