Introduction: An excess of the daily caloric intake facilitates accumulations of fat that leads to the development of obesity. Caloric restriction is the strategy used to restore body composition to optimal levels. Likewise, a period of hypo-nutrition following a balanced diet promotes nutritional deficiencies. Both conditions above described induce changes in the cellular metabolism to which the mitochondria actively participate. These organelles are very dynamic and undergo against continuous fusion and fission processes in correlation with their functionality. Objectives: In this study were monitored the effects of a caloric restriction period, following treatments with normal or hyperlipidic diet, on mitochondrial dynamics in rat liver. Therefore, 4 experimental groups were constituted: N (control diet); HFD high fat diet); Nr (N + restriction); HFDr (HFD + restriction). Attention was focused on the main mitochondrial dynamics’ proteins, monitored by western blot: MFN2 (Mitofusin 2) and OPA1 (Optic atrophy type 1) as fusion markers; DRP1 (Dynamin-related protein 1) as a fission marker. Results: The data show reduction of MFN2 and increase of DRP1 in the Nr group compared to N with variation of the high and low isoforms of OPA1, suggesting increase of the mitochondrial fission. In contrast, both MFN2 and DRP1 increase in the HFDr group compared to HFD. In this case, the data seem to suggest a beneficial effect of the restriction with a probable induction of the mitochondrial biogenesis. This mechanism, in fact, could make more the utilization of lipids mobilized by deposits in consequence to caloric restriction. Conclusions: In conclusion, we can assume that a reduced caloric intake generates reorganization of the mitochondrial liver network by acting differently on the basis of the nutritional conditions prior to the hypocaloric regimen.

Different effect of a period of caloric restriction following treatment with a normal lipid or hyperlipidic diet in the experimental animal model: focus on mitochondrial dynamics proteins in the liver / Migliaccio, V; Sica, R; Lepretti, M; Scudiero, R; Putti, R; Lionetti, L. - In: NMCD. NUTRITION METABOLISM AND CARDIOVASCULAR DISEASES. - ISSN 0939-4753. - 29:8(2019), pp. 882-882.

Different effect of a period of caloric restriction following treatment with a normal lipid or hyperlipidic diet in the experimental animal model: focus on mitochondrial dynamics proteins in the liver.

Scudiero R;
2019

Abstract

Introduction: An excess of the daily caloric intake facilitates accumulations of fat that leads to the development of obesity. Caloric restriction is the strategy used to restore body composition to optimal levels. Likewise, a period of hypo-nutrition following a balanced diet promotes nutritional deficiencies. Both conditions above described induce changes in the cellular metabolism to which the mitochondria actively participate. These organelles are very dynamic and undergo against continuous fusion and fission processes in correlation with their functionality. Objectives: In this study were monitored the effects of a caloric restriction period, following treatments with normal or hyperlipidic diet, on mitochondrial dynamics in rat liver. Therefore, 4 experimental groups were constituted: N (control diet); HFD high fat diet); Nr (N + restriction); HFDr (HFD + restriction). Attention was focused on the main mitochondrial dynamics’ proteins, monitored by western blot: MFN2 (Mitofusin 2) and OPA1 (Optic atrophy type 1) as fusion markers; DRP1 (Dynamin-related protein 1) as a fission marker. Results: The data show reduction of MFN2 and increase of DRP1 in the Nr group compared to N with variation of the high and low isoforms of OPA1, suggesting increase of the mitochondrial fission. In contrast, both MFN2 and DRP1 increase in the HFDr group compared to HFD. In this case, the data seem to suggest a beneficial effect of the restriction with a probable induction of the mitochondrial biogenesis. This mechanism, in fact, could make more the utilization of lipids mobilized by deposits in consequence to caloric restriction. Conclusions: In conclusion, we can assume that a reduced caloric intake generates reorganization of the mitochondrial liver network by acting differently on the basis of the nutritional conditions prior to the hypocaloric regimen.
2019
Different effect of a period of caloric restriction following treatment with a normal lipid or hyperlipidic diet in the experimental animal model: focus on mitochondrial dynamics proteins in the liver / Migliaccio, V; Sica, R; Lepretti, M; Scudiero, R; Putti, R; Lionetti, L. - In: NMCD. NUTRITION METABOLISM AND CARDIOVASCULAR DISEASES. - ISSN 0939-4753. - 29:8(2019), pp. 882-882.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11588/757642
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