There is rising concern for the interaction of environmental contaminants with brain transcriptome and the potential effect on reproductive processes. The present study sought to determine selenoprotein glutathione peroxidase 4 (gpx4) transcriptional activity in the brain and testis of the soil biosentinel, Podarcis sicula, through the main phases of the reproductive cycle and whether pharmaceuticals exert an endocrine disruption. Based on gpx4 cloned amminoacids sequence (GenBank AEX09236.1.), we used a bioinformatic approach to assess the structural role. Specifically, we detected seasonally the reactive oxygen species (ROS) level using electron spin resonance spectroscopy and gpx4 transcriptional activity using qRT-PCR. In addition, the impact of pharmaceuticals was assessed after 21-days of treatment with ICI 182,780 and hCG administration in mating and winter stasis, respectively. Bioinformatic data show gpx4 proteic activity and a phylogenetic profile. ROS contents in lizard brain are significantly less than in testis and display higher levels after treatments. Brain gpx4 expression gives statistically significant seasonal differences, opposite trends in testis and altered expression in both tissues, with evidence of testis morphological and DNA disruption. Taken together, these results provide direct evidence that gpx4 in Podarcis sicula plays a seasonal regulatory role and may be a reliable biomarker for reproductive health toxicity screening.

Seasonal and pharmaceutical-induced changes in selenoprotein glutathione peroxidase 4 activity in the reproductive dynamics of the soil biosentinel Podarcis sicula (Chordata: Reptilia) / Guerriero, Giulia; Parisi, C.; Abdel-Gawad, F. K.; Hentati, O.; D'Errico, Gerardino. - In: MOLECULAR REPRODUCTION AND DEVELOPMENT. - ISSN 1040-452X. - 86:(2019), pp. 1378-1387. [10.1002/mrd.23143]

Seasonal and pharmaceutical-induced changes in selenoprotein glutathione peroxidase 4 activity in the reproductive dynamics of the soil biosentinel Podarcis sicula (Chordata: Reptilia)

Guerriero Giulia
Primo
Project Administration
;
D'Errico Gerardino
2019

Abstract

There is rising concern for the interaction of environmental contaminants with brain transcriptome and the potential effect on reproductive processes. The present study sought to determine selenoprotein glutathione peroxidase 4 (gpx4) transcriptional activity in the brain and testis of the soil biosentinel, Podarcis sicula, through the main phases of the reproductive cycle and whether pharmaceuticals exert an endocrine disruption. Based on gpx4 cloned amminoacids sequence (GenBank AEX09236.1.), we used a bioinformatic approach to assess the structural role. Specifically, we detected seasonally the reactive oxygen species (ROS) level using electron spin resonance spectroscopy and gpx4 transcriptional activity using qRT-PCR. In addition, the impact of pharmaceuticals was assessed after 21-days of treatment with ICI 182,780 and hCG administration in mating and winter stasis, respectively. Bioinformatic data show gpx4 proteic activity and a phylogenetic profile. ROS contents in lizard brain are significantly less than in testis and display higher levels after treatments. Brain gpx4 expression gives statistically significant seasonal differences, opposite trends in testis and altered expression in both tissues, with evidence of testis morphological and DNA disruption. Taken together, these results provide direct evidence that gpx4 in Podarcis sicula plays a seasonal regulatory role and may be a reliable biomarker for reproductive health toxicity screening.
2019
Seasonal and pharmaceutical-induced changes in selenoprotein glutathione peroxidase 4 activity in the reproductive dynamics of the soil biosentinel Podarcis sicula (Chordata: Reptilia) / Guerriero, Giulia; Parisi, C.; Abdel-Gawad, F. K.; Hentati, O.; D'Errico, Gerardino. - In: MOLECULAR REPRODUCTION AND DEVELOPMENT. - ISSN 1040-452X. - 86:(2019), pp. 1378-1387. [10.1002/mrd.23143]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11588/751293
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