Endoparasitoids in the order Hymenoptera are natural enemies of several herbivorous insect pest species. During oviposition they inject a mixture of factors, which include veonm, into the host, ensuring the successful parasitism and the development of their progeny. Although these parasitoid factors are known to be responsible for host manipulation, such as immune system suppression, little is known about both identity and function of the majority of their venom components. To identify the major proteins of Toxoneuron nigriceps (Hymenoptera: Braconidae) venom, we used an integrated transcriptomic and proteomic approach. The tandem-mass spectrometric (LC-MS/MS) data combined with T. nigriceps venom gland transcriptome used as a reference database resulted in the identification of a total of thirty one different proteins. While some of the identified proteins have been described in venom from several parasitoids, others were identified for the first time. Among the identified proteins, hydrolases constituted the most abundant family followed by transferases, oxidoreductases, ligases, lyases and isomerases. The hydrolases identified in the T. nigriceps venom glands included proteases, peptidases and glycosidases, reported as common components of venom from several parasitoid species. Taken together, the identified proteins included factors that could potentially inhibit the host immune system, manipulate host physiological processes and host development, as well as provide nutrients to the parasitoid progeny, degrading host tissues by specific hydrolytic enzymes. The venom decoding provides us with information about the identity of candidate venom factors which could contribute to the success of parasitism, together with other maternal and embryonic factors.

Identification of major Toxoneuron nigriceps venom proteins using an integrated transcriptomic/proteomic approach / Laurino, S; Grossi, Gianfranco; Pucci, Pietro; Flagiello, A; Bufo, Sa; Bianco, G; Salvia, R; Vinson, Sb; Vogel, H; Falabella, P.. - In: INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY. - ISSN 0965-1748. - 76:(2016), pp. 49-61. [10.1016/j.ibmb.2016.07.001]

Identification of major Toxoneuron nigriceps venom proteins using an integrated transcriptomic/proteomic approach.

GROSSI, GIANFRANCO;PUCCI, PIETRO;
2016

Abstract

Endoparasitoids in the order Hymenoptera are natural enemies of several herbivorous insect pest species. During oviposition they inject a mixture of factors, which include veonm, into the host, ensuring the successful parasitism and the development of their progeny. Although these parasitoid factors are known to be responsible for host manipulation, such as immune system suppression, little is known about both identity and function of the majority of their venom components. To identify the major proteins of Toxoneuron nigriceps (Hymenoptera: Braconidae) venom, we used an integrated transcriptomic and proteomic approach. The tandem-mass spectrometric (LC-MS/MS) data combined with T. nigriceps venom gland transcriptome used as a reference database resulted in the identification of a total of thirty one different proteins. While some of the identified proteins have been described in venom from several parasitoids, others were identified for the first time. Among the identified proteins, hydrolases constituted the most abundant family followed by transferases, oxidoreductases, ligases, lyases and isomerases. The hydrolases identified in the T. nigriceps venom glands included proteases, peptidases and glycosidases, reported as common components of venom from several parasitoid species. Taken together, the identified proteins included factors that could potentially inhibit the host immune system, manipulate host physiological processes and host development, as well as provide nutrients to the parasitoid progeny, degrading host tissues by specific hydrolytic enzymes. The venom decoding provides us with information about the identity of candidate venom factors which could contribute to the success of parasitism, together with other maternal and embryonic factors.
2016
Identification of major Toxoneuron nigriceps venom proteins using an integrated transcriptomic/proteomic approach / Laurino, S; Grossi, Gianfranco; Pucci, Pietro; Flagiello, A; Bufo, Sa; Bianco, G; Salvia, R; Vinson, Sb; Vogel, H; Falabella, P.. - In: INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY. - ISSN 0965-1748. - 76:(2016), pp. 49-61. [10.1016/j.ibmb.2016.07.001]
File in questo prodotto:
File Dimensione Formato  
VenomProteinsInsectBiochemMolBiol2016.pdf

accesso aperto

Descrizione: Articolo Principale
Tipologia: Documento in Post-print
Licenza: Dominio pubblico
Dimensione 1.76 MB
Formato Adobe PDF
1.76 MB Adobe PDF Visualizza/Apri

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11588/635768
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 37
  • ???jsp.display-item.citation.isi??? 36
social impact