InYersinia pseudotuberculosiscomplex, the O-antigen of LPS is used for the serological characterization of strains, and 21 serotypes have been identified to date. The O-antigen biosynthesis gene cluster and corresponding O-antigen structure have been described for 18, leaving O:8, O:13 and O:14 unresolved. In this study, two O:8 isolates were examined. The O-antigen gene cluster sequence of strain 151 was near identical to serotype O:4a, though a frame-shift mutation was found inddhD, while No. 6 was different to 151 and carried the O:1b gene cluster. Structural analysis revealed that No. 6 produced a deeply truncated LPS, suggesting a mutation within thewaaFgene. BothddhDandwaaFwere cloned and expressed in 151 and No. 6 strains, respectively, and it appeared that expression ofddhDgene in strain 151 restored the O-antigen on LPS, whilewaaFin No. 6 resulted in an LPS truncated less severely but still without the O-antigen, suggesting that other mutations occurred in this strain. Thus, both O:8 isolates were found to be spontaneous O-antigen-negative mutants derived from other validated serotypes, and we propose to remove this serotype from the O-serotyping scheme, as the O:8 serological specificity is not based on the O-antigen.
Serotype O:8 isolates in the Yersinia pseudotuberculosis complex have different O-antigen gene clusters and produce various forms of rough LPS / Kenyon, Johanna J; Duda, Katarzyna A; DE FELICE, Antonia; Cunneen, Monica M; Molinaro, Antonio; Laitinen, Juha; Skurnik, Mikael; Holst, Otto; Reeves, Peter R; DE CASTRO, Cristina. - In: INNATE IMMUNITY. - ISSN 1753-4259. - 22:3(2016), pp. 205-217. [10.1177/1753425916631403]
Serotype O:8 isolates in the Yersinia pseudotuberculosis complex have different O-antigen gene clusters and produce various forms of rough LPS
DE FELICE, ANTONIA;MOLINARO, ANTONIO;DE CASTRO, CRISTINA
2016
Abstract
InYersinia pseudotuberculosiscomplex, the O-antigen of LPS is used for the serological characterization of strains, and 21 serotypes have been identified to date. The O-antigen biosynthesis gene cluster and corresponding O-antigen structure have been described for 18, leaving O:8, O:13 and O:14 unresolved. In this study, two O:8 isolates were examined. The O-antigen gene cluster sequence of strain 151 was near identical to serotype O:4a, though a frame-shift mutation was found inddhD, while No. 6 was different to 151 and carried the O:1b gene cluster. Structural analysis revealed that No. 6 produced a deeply truncated LPS, suggesting a mutation within thewaaFgene. BothddhDandwaaFwere cloned and expressed in 151 and No. 6 strains, respectively, and it appeared that expression ofddhDgene in strain 151 restored the O-antigen on LPS, whilewaaFin No. 6 resulted in an LPS truncated less severely but still without the O-antigen, suggesting that other mutations occurred in this strain. Thus, both O:8 isolates were found to be spontaneous O-antigen-negative mutants derived from other validated serotypes, and we propose to remove this serotype from the O-serotyping scheme, as the O:8 serological specificity is not based on the O-antigen.File | Dimensione | Formato | |
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