Steroids are a class of hormones improperly used in livestock as growth-promoting agents.  Due to their high risk for human health, the European Union (EU) has strictly forbidden the administration of all natural and synthetic steroid hormones to food-producing animals, and the development of new rapid detection methods are greatly encouraged.  This work reports a novel fluorescence polarization assay, ready to use, capable of detecting 17β-estradiol directly in milk samples with a low limit of detection of <10 pmol.  It is based on the coupling of monospecific antibodies against 17β-estradiol and fluorophores, capable of modulating the fluorescence polarization emission on the basis of the specific binding of antibodies to fluorescence-labeled 17β-estradiol deriv.  The successful detection of 17β-estradiol has disclosed the development of an efficient method, easily extensible to any food matrix and having the potential to become a milestone in food quality and safety.

A Fluorescence Polarization Assay to Detect Steroid Hormone Traces in Milk / Varriale, Antonio; Pennacchio, Anna; Pinto, Gabriella; Oliviero, Giorgia; D'Errico, Stefano; Majoli, Adelia; Scala, Andrea; Capo, Alessandro; Pennacchio, Angela; Di Giovanni, Stefano; Staiano, Maria; D'Auria, Sabato. - In: JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY. - ISSN 0021-8561. - 63:41(2015), pp. 9159-9164. [10.1021/acs.jafc.5b03689]

A Fluorescence Polarization Assay to Detect Steroid Hormone Traces in Milk

PINTO, GABRIELLA;OLIVIERO, GIORGIA;D'ERRICO, STEFANO;SCALA, ANDREA;D'AURIA, SABATO
2015

Abstract

Steroids are a class of hormones improperly used in livestock as growth-promoting agents.  Due to their high risk for human health, the European Union (EU) has strictly forbidden the administration of all natural and synthetic steroid hormones to food-producing animals, and the development of new rapid detection methods are greatly encouraged.  This work reports a novel fluorescence polarization assay, ready to use, capable of detecting 17β-estradiol directly in milk samples with a low limit of detection of <10 pmol.  It is based on the coupling of monospecific antibodies against 17β-estradiol and fluorophores, capable of modulating the fluorescence polarization emission on the basis of the specific binding of antibodies to fluorescence-labeled 17β-estradiol deriv.  The successful detection of 17β-estradiol has disclosed the development of an efficient method, easily extensible to any food matrix and having the potential to become a milestone in food quality and safety.
2015
A Fluorescence Polarization Assay to Detect Steroid Hormone Traces in Milk / Varriale, Antonio; Pennacchio, Anna; Pinto, Gabriella; Oliviero, Giorgia; D'Errico, Stefano; Majoli, Adelia; Scala, Andrea; Capo, Alessandro; Pennacchio, Angela; Di Giovanni, Stefano; Staiano, Maria; D'Auria, Sabato. - In: JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY. - ISSN 0021-8561. - 63:41(2015), pp. 9159-9164. [10.1021/acs.jafc.5b03689]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11588/625025
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