Abstract Sulfolobus spindle-shaped virus 1 represents a model for studying virus-host interaction in harsh environments, and it is so far the only member of the family Fuselloviridae that shows a UV-inducible life cycle. Although the virus has been extensively studied, mechanisms underpinning the maintenance of lysogeny as well as those regulating the UV induction have received little attention. Recently, a novel SSV1 transcription factor, F55, was identified. This factor was able to bind in vitro to several sequences derived from the early and UV-inducible promoters of the SSV1 genome. The location of these binding sites together with the differential affinity of F55 for these sequences led to the hypothesis that this protein might be involved in the maintenance of the SSV1 lysogeny. Here, we report an in vivo survey of the molecular events occurring at the UV-inducible region of the SSV1 genome, with a focus on the binding profile of F55 before and after the UV irradiation. The binding of F55 to the target promoters correlates with transcription repression, whereas its dissociation is paralleled by transcription activation. Therefore, we propose that F55 acts as a molecular switch for the transcriptional regulation of the early viral genes.

Unravelling the role of the F55 regulator in the transition from lysogeny to UV induction of Sulfolobus spindle-shaped virus 1 / Fusco, Salvatore; She, Qunxin; Fiorentino, Gabriella; Bartolucci, Simonetta; Contursi, Patrizia. - In: JOURNAL OF VIROLOGY. - ISSN 0022-538X. - 89:12(2015), pp. 6453-6461. [10.1128/JVI.00363-15]

Unravelling the role of the F55 regulator in the transition from lysogeny to UV induction of Sulfolobus spindle-shaped virus 1

FUSCO, SALVATORE;FIORENTINO, GABRIELLA;BARTOLUCCI, SIMONETTA;CONTURSI, PATRIZIA
2015

Abstract

Abstract Sulfolobus spindle-shaped virus 1 represents a model for studying virus-host interaction in harsh environments, and it is so far the only member of the family Fuselloviridae that shows a UV-inducible life cycle. Although the virus has been extensively studied, mechanisms underpinning the maintenance of lysogeny as well as those regulating the UV induction have received little attention. Recently, a novel SSV1 transcription factor, F55, was identified. This factor was able to bind in vitro to several sequences derived from the early and UV-inducible promoters of the SSV1 genome. The location of these binding sites together with the differential affinity of F55 for these sequences led to the hypothesis that this protein might be involved in the maintenance of the SSV1 lysogeny. Here, we report an in vivo survey of the molecular events occurring at the UV-inducible region of the SSV1 genome, with a focus on the binding profile of F55 before and after the UV irradiation. The binding of F55 to the target promoters correlates with transcription repression, whereas its dissociation is paralleled by transcription activation. Therefore, we propose that F55 acts as a molecular switch for the transcriptional regulation of the early viral genes.
2015
Unravelling the role of the F55 regulator in the transition from lysogeny to UV induction of Sulfolobus spindle-shaped virus 1 / Fusco, Salvatore; She, Qunxin; Fiorentino, Gabriella; Bartolucci, Simonetta; Contursi, Patrizia. - In: JOURNAL OF VIROLOGY. - ISSN 0022-538X. - 89:12(2015), pp. 6453-6461. [10.1128/JVI.00363-15]
File in questo prodotto:
File Dimensione Formato  
Unravelling the Role of the F55 Regulator in the Transition from Lysogeny to UV Induction of Sulfolobus Spindle-Shaped Virus 1.pdf

solo utenti autorizzati

Descrizione: pdf lavoro
Licenza: Accesso privato/ristretto
Dimensione 1.21 MB
Formato Adobe PDF
1.21 MB Adobe PDF   Visualizza/Apri   Richiedi una copia

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11588/619111
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 22
  • ???jsp.display-item.citation.isi??? 21
social impact