INTRODUCTION: Nicoletella semolina is gram-negative bacterium recently described as member of the Pasteurellaceae family(1). N. semolina has been isolated from horses with respiratory disorders (2) however it is still unclear if this bacterium plays a role as potential pathogen, opportunistic or just as commensal of the normal bacteriological flora. To the best of authors’ knowledge there are no descriptions of the isolation of this bacterium in donkeys. The aim of the present report is to describe, for the first time, the isolation of N. semolina in a donkey foal from Italy. MATERIALS AND METHODS: Three 2-3-month-old crossbreed donkey carcass (A-B-C) were submitted to the necropsy. Anamnesis reported severe respiratory disorders before death. At necropsy in all foals was reported generalized pneumonia associated with purulent-catarrhal collection and emphysema diffuse; the foal A showed lesions in all lung lobes, pericardial effusion and subsequent cardiac tamponade. Samples were collected from lungs and were plated onto two Blood Agar Base and MacConkey agar SS plates, and incubated aerobically and in microaerophilia at 37°C±1°C for 72h. The plates were observed every 24h. On all isolates were performed identification using the Vitek 2 Compact system (bioMérieux). On the strain isolated from the foal A was performed the sequencing and analysis of 16S rRNA genes. PCR was performed with MicroSeq 500 rDNA PCR Kit (Life Thecnoligies). Amplification products of the 16SrRNA genes were used for cycle sequencing with MicroSeq 500 rDNA Sequencing Kit. Sequence analysis and alignment were carried out using SeqScape v.2.5 (Applied Biosystem) and NCBI/BLAST/blastn suite. RESULTS: After 24h of incubation aerobic bacterial cultures of all 3 foals (A-B-C) in Blood Agar Base yielded heavy growth of Streptococcus equi subsp. zooepidemicus; at the same time point in MacConkey agar plates (foal C) was detected the co-presence of Salmonella kasenyi. After 48h of incubation in Blood Agar Base plates (foal B) was isolated Nocardia spp. Furthermore at 72h in in Blood Agar Base plates (foal A) was detected the appearance of colonies gray, rounded, waxy, nonhaemolytic, nonadherent, similar to a grain of semolina wheat (1). The organism was identified as a pleomorphic gram-negative coccobacillus, nonmotile, catalase and oxidase positive and negative for indole test. 16S rDNA sequencing revealed 99% homology with N. semolina. DISCUSSION AND CONCLUSIONS: The present report described in the donkey is fairly similar to that noted recently in young horses (3) where is described the isolation of N. semolina associated with Streptococcus equi subsp. zooepidemicus with respiratory disorders (2). In the donkey is still be determined whether N. semolina is a primary pathogen or a commensal which can occasionally cause disease. Further studies are required to clarify the potential pathogen of this bacterium in the donkeys. REFERENCES: 1.Kuhnert P., Korczak B., Falsen E., Straub R., Hoops A., Boerlin P., Frey J., Mutters R., 2004. Nicoletella semolina gen. nov., sp. nov., a New Member of Pasteurellaceae Isolated from Horses with Airway Disease.

Nicoletella semolina in a donkey: first case report from Italy / Nizza, S.; Vecchio, D.; Martucciello, A.; Alfano, D.; Veneziano, Vincenzo; Corrado, F.; De Roma, A.; Scognamiglio, R.; De Carlo, E.. - 1:1(2014), pp. 119-119. (Intervento presentato al convegno 3rd European EAVLD Congress - tenutosi a Pisa (Italy) nel October 12-15, 2014).

Nicoletella semolina in a donkey: first case report from Italy

VENEZIANO, VINCENZO;
2014

Abstract

INTRODUCTION: Nicoletella semolina is gram-negative bacterium recently described as member of the Pasteurellaceae family(1). N. semolina has been isolated from horses with respiratory disorders (2) however it is still unclear if this bacterium plays a role as potential pathogen, opportunistic or just as commensal of the normal bacteriological flora. To the best of authors’ knowledge there are no descriptions of the isolation of this bacterium in donkeys. The aim of the present report is to describe, for the first time, the isolation of N. semolina in a donkey foal from Italy. MATERIALS AND METHODS: Three 2-3-month-old crossbreed donkey carcass (A-B-C) were submitted to the necropsy. Anamnesis reported severe respiratory disorders before death. At necropsy in all foals was reported generalized pneumonia associated with purulent-catarrhal collection and emphysema diffuse; the foal A showed lesions in all lung lobes, pericardial effusion and subsequent cardiac tamponade. Samples were collected from lungs and were plated onto two Blood Agar Base and MacConkey agar SS plates, and incubated aerobically and in microaerophilia at 37°C±1°C for 72h. The plates were observed every 24h. On all isolates were performed identification using the Vitek 2 Compact system (bioMérieux). On the strain isolated from the foal A was performed the sequencing and analysis of 16S rRNA genes. PCR was performed with MicroSeq 500 rDNA PCR Kit (Life Thecnoligies). Amplification products of the 16SrRNA genes were used for cycle sequencing with MicroSeq 500 rDNA Sequencing Kit. Sequence analysis and alignment were carried out using SeqScape v.2.5 (Applied Biosystem) and NCBI/BLAST/blastn suite. RESULTS: After 24h of incubation aerobic bacterial cultures of all 3 foals (A-B-C) in Blood Agar Base yielded heavy growth of Streptococcus equi subsp. zooepidemicus; at the same time point in MacConkey agar plates (foal C) was detected the co-presence of Salmonella kasenyi. After 48h of incubation in Blood Agar Base plates (foal B) was isolated Nocardia spp. Furthermore at 72h in in Blood Agar Base plates (foal A) was detected the appearance of colonies gray, rounded, waxy, nonhaemolytic, nonadherent, similar to a grain of semolina wheat (1). The organism was identified as a pleomorphic gram-negative coccobacillus, nonmotile, catalase and oxidase positive and negative for indole test. 16S rDNA sequencing revealed 99% homology with N. semolina. DISCUSSION AND CONCLUSIONS: The present report described in the donkey is fairly similar to that noted recently in young horses (3) where is described the isolation of N. semolina associated with Streptococcus equi subsp. zooepidemicus with respiratory disorders (2). In the donkey is still be determined whether N. semolina is a primary pathogen or a commensal which can occasionally cause disease. Further studies are required to clarify the potential pathogen of this bacterium in the donkeys. REFERENCES: 1.Kuhnert P., Korczak B., Falsen E., Straub R., Hoops A., Boerlin P., Frey J., Mutters R., 2004. Nicoletella semolina gen. nov., sp. nov., a New Member of Pasteurellaceae Isolated from Horses with Airway Disease.
2014
Nicoletella semolina in a donkey: first case report from Italy / Nizza, S.; Vecchio, D.; Martucciello, A.; Alfano, D.; Veneziano, Vincenzo; Corrado, F.; De Roma, A.; Scognamiglio, R.; De Carlo, E.. - 1:1(2014), pp. 119-119. (Intervento presentato al convegno 3rd European EAVLD Congress - tenutosi a Pisa (Italy) nel October 12-15, 2014).
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11588/619077
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