The present paper reports the characterization of ABE (acetone-butanol-ethanol) production by Clostridium acetobutylicum DSM 792 for sugars representative of hydrolysed lignocellulosic biomass (glucose, mannose, arabinose, xylose). The attention was focused on: the selection of an optimal medium for the simultaneous conversion of the investigated sugars; the assessment of interference-synergistic effects during the fermentation of mixtures of the investigated sugars. The synthetic medium was optimised in terms of nutritional factors: the KH2PO4-K2HPO4 concentration was increased up to 5 g/L; the MgSO4 concentration was increased up to 2 g/L; the MnSO4 concentration was increased up to 0.1 g/L; the FeSO4 concentration ranged between 0.002 and 0.01 g/L); the CaCO3 concentration was increased up to 10 g/L. The optimal concentration of the investigated factors was assessed and it varied from one sugar to another. The batch fermentations of a mixture of the four sugars highlighted their synergistic effects. Once set the initial concentration of the sugars (60 g/L), the butanol and solvent concentration increased up to 14.6 and 20.6 g/L, respectively, when the four sugars were present.

Butanol production from hexoses and pentoses by fermentation of Clostridium acetobutylicum / Raganati, Francesca; Olivieri, Giuseppe; Götz, Peter; Marzocchella, Antonio; Salatino, Piero. - In: ANAEROBE. - ISSN 1075-9964. - 34:(2015), pp. 146-155. [10.1016/j.anaerobe.2015.05.008]

Butanol production from hexoses and pentoses by fermentation of Clostridium acetobutylicum

RAGANATI, FRANCESCA;OLIVIERI, GIUSEPPE;MARZOCCHELLA, ANTONIO;SALATINO, PIERO
2015

Abstract

The present paper reports the characterization of ABE (acetone-butanol-ethanol) production by Clostridium acetobutylicum DSM 792 for sugars representative of hydrolysed lignocellulosic biomass (glucose, mannose, arabinose, xylose). The attention was focused on: the selection of an optimal medium for the simultaneous conversion of the investigated sugars; the assessment of interference-synergistic effects during the fermentation of mixtures of the investigated sugars. The synthetic medium was optimised in terms of nutritional factors: the KH2PO4-K2HPO4 concentration was increased up to 5 g/L; the MgSO4 concentration was increased up to 2 g/L; the MnSO4 concentration was increased up to 0.1 g/L; the FeSO4 concentration ranged between 0.002 and 0.01 g/L); the CaCO3 concentration was increased up to 10 g/L. The optimal concentration of the investigated factors was assessed and it varied from one sugar to another. The batch fermentations of a mixture of the four sugars highlighted their synergistic effects. Once set the initial concentration of the sugars (60 g/L), the butanol and solvent concentration increased up to 14.6 and 20.6 g/L, respectively, when the four sugars were present.
2015
Butanol production from hexoses and pentoses by fermentation of Clostridium acetobutylicum / Raganati, Francesca; Olivieri, Giuseppe; Götz, Peter; Marzocchella, Antonio; Salatino, Piero. - In: ANAEROBE. - ISSN 1075-9964. - 34:(2015), pp. 146-155. [10.1016/j.anaerobe.2015.05.008]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11588/613107
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