The structure and the immunology of the lipopolysaccharide (LPS) of Pseudomonas entomophila, an entomopathogenic bacterium isolated from the fruit fly Drosophila melanogaster, was characterized. The O-antigen portion was established and resulted to be built up of a repetitive unit constituted by four monosaccharide residues, all L configured, all deoxy at C-6 and with an acetamido function at C-2: /3)-a-L-FucNAc-(1/4)-a-L-FucNAc-(1/3)-a-L-FucNAc-(1/3)-b-L-QuiNAc-(1/ The structural analysis of lipid A, showed a mixture of different species. The diphosphorylated glucosamine backbone carries six fatty acids consistent with the composition C10:0 3(OH), C12:0 2(OH) and C12:0 3(OH), whereas other species differs by the number of phosphates and/or of fatty acids. The immunology experiments demonstrated that the LPS structure of P. entomophila displayed a low ability to engage the TLR4-mediated signaling correlated to a significant antagonistic activity toward hexaacylated LPS structures

Determination of the structure of the O-antigen and the lipid A from the entomopathogenic bacterium Pseudomonas entomophila lipopolysaccharide along with its immunological properties / Speciale, Immacolata; Ida, Paciello; Luigi Lembo, Fazio; Luisa, Sturiale; Angelo, Palmigiano; Lanzetta, Rosa; Parrilli, Michelangelo; Domenico, Garozzo; Bruno, Lemaitre; Maria Lina, Bernardini; Molinaro, Antonio; DE CASTRO, Cristina. - In: CARBOHYDRATE RESEARCH. - ISSN 0008-6215. - 412:(2015), pp. 20-27. [10.1016/j.carres.2015.04.017]

Determination of the structure of the O-antigen and the lipid A from the entomopathogenic bacterium Pseudomonas entomophila lipopolysaccharide along with its immunological properties

SPECIALE, IMMACOLATA;LANZETTA, ROSA;PARRILLI, MICHELANGELO;MOLINARO, ANTONIO;DE CASTRO, CRISTINA
2015

Abstract

The structure and the immunology of the lipopolysaccharide (LPS) of Pseudomonas entomophila, an entomopathogenic bacterium isolated from the fruit fly Drosophila melanogaster, was characterized. The O-antigen portion was established and resulted to be built up of a repetitive unit constituted by four monosaccharide residues, all L configured, all deoxy at C-6 and with an acetamido function at C-2: /3)-a-L-FucNAc-(1/4)-a-L-FucNAc-(1/3)-a-L-FucNAc-(1/3)-b-L-QuiNAc-(1/ The structural analysis of lipid A, showed a mixture of different species. The diphosphorylated glucosamine backbone carries six fatty acids consistent with the composition C10:0 3(OH), C12:0 2(OH) and C12:0 3(OH), whereas other species differs by the number of phosphates and/or of fatty acids. The immunology experiments demonstrated that the LPS structure of P. entomophila displayed a low ability to engage the TLR4-mediated signaling correlated to a significant antagonistic activity toward hexaacylated LPS structures
2015
Determination of the structure of the O-antigen and the lipid A from the entomopathogenic bacterium Pseudomonas entomophila lipopolysaccharide along with its immunological properties / Speciale, Immacolata; Ida, Paciello; Luigi Lembo, Fazio; Luisa, Sturiale; Angelo, Palmigiano; Lanzetta, Rosa; Parrilli, Michelangelo; Domenico, Garozzo; Bruno, Lemaitre; Maria Lina, Bernardini; Molinaro, Antonio; DE CASTRO, Cristina. - In: CARBOHYDRATE RESEARCH. - ISSN 0008-6215. - 412:(2015), pp. 20-27. [10.1016/j.carres.2015.04.017]
File in questo prodotto:
File Dimensione Formato  
105_entomophila.pdf

solo utenti autorizzati

Tipologia: Altro materiale allegato
Licenza: Accesso privato/ristretto
Dimensione 1.4 MB
Formato Adobe PDF
1.4 MB Adobe PDF   Visualizza/Apri   Richiedi una copia

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11588/611286
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 6
  • ???jsp.display-item.citation.isi??? 6
social impact