The effects of organic system on Stearoyl-CoA desaturase (SCD) gene expression in milk somatic cells was evaluated in 30 pregnant pluriparous goats, divided into two homoge- neous groups (O and S). Group O was fed according to EC Regulation 834/2007 and led to pasture while group S was stabled and received alfalfa hay as forage. After kids wean- ing, milk yield was recorded and individual representative milk samples were analysed for chemical composition and fatty acid profile. The SCD gene expression was studied by extraction of mRNA from milk somatic cells and RT-PCR analysis. The body weight of goats of both groups did not show any significant change during the trial. Average milk yield did not differ statistically between the groups while milk from group O showed a significantly higher fat content than those from group S (65.9 vs. 54.3g/day, P<0.01). Organic system significantly affected the percentages of c9 C18:1, t11 C18:1, octadecadienoic acid, octade- catrienoic acid, monounsaturated fatty acids as well as the c9 t11 conjugated linoleic acid (CLA) (0.810 vs. 0.542g/100g of fat, for groups O and S, respectively, P<0.01) andCLA (0.860 vs. 0.580g/100g of fat for groups O and S, respectively, P<0.01) concentrations in milk. The Stearoyl-CoA desaturase ratios were not different between treatments. Finally, the SCD gene expression was significantly higher in the somatic cells in milk yielded from the “organic” group (AU:1.5±0.5 vs. 0.39±0.11 for groups O and S, respectively, P<0.01).
Influence of organic system on stearoyl-CoA desaturase gene expression in goat milk / Tudisco, Raffaella; Calabro', Serena; Cutrignelli, MONICA ISABELLA; Moniello, G.; Grossi, Micaela; Gonzalez, O. J.; Piccolo, V.; Infascelli, Federico. - In: SMALL RUMINANT RESEARCH. - ISSN 0921-4488. - 106S:(2012), pp. S37-S42. [10.1016/j.smallrumres.2012.04.031]
Influence of organic system on stearoyl-CoA desaturase gene expression in goat milk
TUDISCO, RAFFAELLA;CALABRO', SERENA;CUTRIGNELLI, MONICA ISABELLA;GROSSI, MICAELA;INFASCELLI, FEDERICO
2012
Abstract
The effects of organic system on Stearoyl-CoA desaturase (SCD) gene expression in milk somatic cells was evaluated in 30 pregnant pluriparous goats, divided into two homoge- neous groups (O and S). Group O was fed according to EC Regulation 834/2007 and led to pasture while group S was stabled and received alfalfa hay as forage. After kids wean- ing, milk yield was recorded and individual representative milk samples were analysed for chemical composition and fatty acid profile. The SCD gene expression was studied by extraction of mRNA from milk somatic cells and RT-PCR analysis. The body weight of goats of both groups did not show any significant change during the trial. Average milk yield did not differ statistically between the groups while milk from group O showed a significantly higher fat content than those from group S (65.9 vs. 54.3g/day, P<0.01). Organic system significantly affected the percentages of c9 C18:1, t11 C18:1, octadecadienoic acid, octade- catrienoic acid, monounsaturated fatty acids as well as the c9 t11 conjugated linoleic acid (CLA) (0.810 vs. 0.542g/100g of fat, for groups O and S, respectively, P<0.01) andCLA (0.860 vs. 0.580g/100g of fat for groups O and S, respectively, P<0.01) concentrations in milk. The Stearoyl-CoA desaturase ratios were not different between treatments. Finally, the SCD gene expression was significantly higher in the somatic cells in milk yielded from the “organic” group (AU:1.5±0.5 vs. 0.39±0.11 for groups O and S, respectively, P<0.01).File | Dimensione | Formato | |
---|---|---|---|
Tudisco et al. 2012 SRR.pdf
accesso aperto
Descrizione: Paper
Tipologia:
Documento in Post-print
Licenza:
Dominio pubblico
Dimensione
216.35 kB
Formato
Adobe PDF
|
216.35 kB | Adobe PDF | Visualizza/Apri |
I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.