Background: Less than 30% of malignant gliomas respond to adjuvant chemotherapy. Here, we have asked whether variations in the constitutive expression of early-growth response factor 1 (EGR-1) predicted acute cytotoxicity and clonogenic cell death in vitro, induced by six different chemotherapics. Materials and methods: Cytotoxicity assays were performed on cells derived from fresh tumor explants of 18 human cases of malignant glioma. In addition to EGR-1, tumor cultures were investigated for genetic alterations and the expression of cancer regulating factors, related to the p53 pathway. Results: We found that sensitivity to cisplatin correlates significantly with levels of EGR-1 expression in tumors with wild-type p53/INK4a/p16 status. Conclusion: Increased knowledge of the mechanisms regulating EGR-1 expression in wild-type p53/INK4a/p16 cases of glioma may help in the design of new chemotherapeutic strategies for these tumors.

Sensitivity to cisplatin in primary cell lines derived from human glioma correlates with levels of EGR-1 expression / Calogero, A.; Porcellini, Antonio; Lombari, V.; Fabbiano, C.; Arcella, A.; Miscusi, M.; Ponti, D.; Ragona, G.. - In: CANCER CELL INTERNATIONAL. - ISSN 1475-2867. - 11:5(2011), pp. 1-11. [10.1186/1475-2867-11-5]

Sensitivity to cisplatin in primary cell lines derived from human glioma correlates with levels of EGR-1 expression.

PORCELLINI, ANTONIO;
2011

Abstract

Background: Less than 30% of malignant gliomas respond to adjuvant chemotherapy. Here, we have asked whether variations in the constitutive expression of early-growth response factor 1 (EGR-1) predicted acute cytotoxicity and clonogenic cell death in vitro, induced by six different chemotherapics. Materials and methods: Cytotoxicity assays were performed on cells derived from fresh tumor explants of 18 human cases of malignant glioma. In addition to EGR-1, tumor cultures were investigated for genetic alterations and the expression of cancer regulating factors, related to the p53 pathway. Results: We found that sensitivity to cisplatin correlates significantly with levels of EGR-1 expression in tumors with wild-type p53/INK4a/p16 status. Conclusion: Increased knowledge of the mechanisms regulating EGR-1 expression in wild-type p53/INK4a/p16 cases of glioma may help in the design of new chemotherapeutic strategies for these tumors.
2011
Sensitivity to cisplatin in primary cell lines derived from human glioma correlates with levels of EGR-1 expression / Calogero, A.; Porcellini, Antonio; Lombari, V.; Fabbiano, C.; Arcella, A.; Miscusi, M.; Ponti, D.; Ragona, G.. - In: CANCER CELL INTERNATIONAL. - ISSN 1475-2867. - 11:5(2011), pp. 1-11. [10.1186/1475-2867-11-5]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11588/379968
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