We had previously detected a transforming oncogene, designated PTC, in 25% of 20 papillary thyroid carcinomas. In order to characterize further the transforming activity of this tumour histotype, a new panel of tumour specimens from 16 patients was analysed by using a modified calcium phosphate-DNA coprecipitation transfection protocol. Tumour DNA from 10 patients (62%) displayed a transforming activity due to activation of three different oncogenes identified in four cases as PTC, in four cases as TRK, and in two cases as N-RAS. The same structural alterations of PTC and TRK (gene rearrangements) as well as of N-RAS (point mutation) detected in the NIH3T3 transformants, were also found in the original tumour DNAs, thus indicating that their activation was not due to transfection procedures. Since both PTC, a novel rearranged form of RET, and TRK display a tyrosine protein kinase activity, it is proposed that the activation of this class of oncogenes is specifically involved in the pathogenesis of papillary thyroid cancer.

High frequency of activation of tyrosine kinase oncogenes in human papillary thyroid carcinoma / Bongarzone, I.; Pierotti, M.; Monzini, N.; Mondellini, P.; Manenti, G.; Donghi, R.; Pilotti, S.; Grieco, M.; Santoro, Massimo; Fusco, Alfredo; Vecchio, Giancarlo; Della Porta, G.. - In: ONCOGENE. - ISSN 0950-9232. - STAMPA. - 4:(1989), pp. 1457-1462.

High frequency of activation of tyrosine kinase oncogenes in human papillary thyroid carcinoma

SANTORO, MASSIMO;FUSCO, ALFREDO;VECCHIO, GIANCARLO;
1989

Abstract

We had previously detected a transforming oncogene, designated PTC, in 25% of 20 papillary thyroid carcinomas. In order to characterize further the transforming activity of this tumour histotype, a new panel of tumour specimens from 16 patients was analysed by using a modified calcium phosphate-DNA coprecipitation transfection protocol. Tumour DNA from 10 patients (62%) displayed a transforming activity due to activation of three different oncogenes identified in four cases as PTC, in four cases as TRK, and in two cases as N-RAS. The same structural alterations of PTC and TRK (gene rearrangements) as well as of N-RAS (point mutation) detected in the NIH3T3 transformants, were also found in the original tumour DNAs, thus indicating that their activation was not due to transfection procedures. Since both PTC, a novel rearranged form of RET, and TRK display a tyrosine protein kinase activity, it is proposed that the activation of this class of oncogenes is specifically involved in the pathogenesis of papillary thyroid cancer.
1989
High frequency of activation of tyrosine kinase oncogenes in human papillary thyroid carcinoma / Bongarzone, I.; Pierotti, M.; Monzini, N.; Mondellini, P.; Manenti, G.; Donghi, R.; Pilotti, S.; Grieco, M.; Santoro, Massimo; Fusco, Alfredo; Vecchio, Giancarlo; Della Porta, G.. - In: ONCOGENE. - ISSN 0950-9232. - STAMPA. - 4:(1989), pp. 1457-1462.
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11588/373201
Citazioni
  • ???jsp.display-item.citation.pmc??? 61
  • Scopus ND
  • ???jsp.display-item.citation.isi??? ND
social impact