The identification and characterization of a mouse tRNA(Trp) pseudogene is reported. A synthetic oligonucleotide (31 mer), identical with the 3' half of a tRNA(Trp), was used to examine three mouse lambda clones that are known to contain clusters of tRNA genes. A fragment of one of these lambda clones strongly hybridizes to the oligonucleotide; the sequence of this region shows the presence of a gene significantly homologous to the chick tRNA(Trp). However two point mutations and a three base deletion prevent the folding of a possible transcript of this gene. The presence of a conserved promoter sequence for RNA polymerase III, that should allow the transcription of this gene, does not ensure the transcription of the gene, at least in our in vitro system.

A mouse tRNA pseudogene derived from a tRNATrp coding sequence / Ammendola, Rosario; Russo, Tommaso; Cimino, Filiberto. - In: ITALIAN JOURNAL OF BIOCHEMISTRY. - ISSN 0021-2938. - STAMPA. - 37:(1988), pp. 85-90.

A mouse tRNA pseudogene derived from a tRNATrp coding sequence

AMMENDOLA, ROSARIO;RUSSO, TOMMASO;CIMINO, FILIBERTO
1988

Abstract

The identification and characterization of a mouse tRNA(Trp) pseudogene is reported. A synthetic oligonucleotide (31 mer), identical with the 3' half of a tRNA(Trp), was used to examine three mouse lambda clones that are known to contain clusters of tRNA genes. A fragment of one of these lambda clones strongly hybridizes to the oligonucleotide; the sequence of this region shows the presence of a gene significantly homologous to the chick tRNA(Trp). However two point mutations and a three base deletion prevent the folding of a possible transcript of this gene. The presence of a conserved promoter sequence for RNA polymerase III, that should allow the transcription of this gene, does not ensure the transcription of the gene, at least in our in vitro system.
1988
A mouse tRNA pseudogene derived from a tRNATrp coding sequence / Ammendola, Rosario; Russo, Tommaso; Cimino, Filiberto. - In: ITALIAN JOURNAL OF BIOCHEMISTRY. - ISSN 0021-2938. - STAMPA. - 37:(1988), pp. 85-90.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11588/373098
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