The transcription start site and promoter of the rat gene coding for the transcription factor NF-1 have been identified. The NF-1 promoter was fused to the chloramphenicol acetyltransferase-coding sequence, and the resulting plasmid was transcriptionally active in the HepG2 cell line. Footprinting and gel retardation analysis indicated that the transcription factor Sp1 binds to the NF-1 promoter. Mutants in the Sp1-binding site displayed a strong reduction in transcriptional activity.

Transcription of the promoter of the rat NF-1 gene depends on the integrity of an Sp1 recognition site / Ammendola, Rosario; F., Gounari; G., Piaggio; DE SIMONE, Vincenzo; Cortese, Riccardo. - In: MOLECULAR AND CELLULAR BIOLOGY. - ISSN 0270-7306. - STAMPA. - 10:(1990), pp. 387-390.

Transcription of the promoter of the rat NF-1 gene depends on the integrity of an Sp1 recognition site

AMMENDOLA, ROSARIO;DE SIMONE, VINCENZO;CORTESE, RICCARDO
1990

Abstract

The transcription start site and promoter of the rat gene coding for the transcription factor NF-1 have been identified. The NF-1 promoter was fused to the chloramphenicol acetyltransferase-coding sequence, and the resulting plasmid was transcriptionally active in the HepG2 cell line. Footprinting and gel retardation analysis indicated that the transcription factor Sp1 binds to the NF-1 promoter. Mutants in the Sp1-binding site displayed a strong reduction in transcriptional activity.
1990
Transcription of the promoter of the rat NF-1 gene depends on the integrity of an Sp1 recognition site / Ammendola, Rosario; F., Gounari; G., Piaggio; DE SIMONE, Vincenzo; Cortese, Riccardo. - In: MOLECULAR AND CELLULAR BIOLOGY. - ISSN 0270-7306. - STAMPA. - 10:(1990), pp. 387-390.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11588/373092
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