A cDNA minilibrary in pUC18 has been generated from 3-month-old rat brains. Two hundred clones were randomly selected and sequenced. Comparison with a nucleic acid and protein data bank revealed a number of cDNA fragments not homologous to any published sequence. Northern blot analyses of some of these clones yielded 5 brain-specific cDNA fragments. The full-length cDNA for one of these clones has been isolated and completely sequenced. It corresponds to an mRNA of about 1,500 nucleotides, which is present in brain and, to a lesser extent, in heart and skeletal muscle. Its expression is developmentally regulated in the rat brain from 14-day embryos to 3-month-old adults. A very recent comparison with the EMBL nucleotide sequence data bank showed that this mRNA codes for a brain-specific snRNP-associated protein.
Isolation of cDNA fragments hybridizing to rat brain specific mRNAs / Esposito, Franca; Ammendola, Rosario; Duilio, Angela; F., Costanzo; M., Giordano; Zambrano, Nicola; P., D'Agostino; Russo, Tommaso; Cimino, Filiberto. - In: DEVELOPMENTAL NEUROSCIENCE. - ISSN 0378-5866. - STAMPA. - 12:(1990), pp. 373-391.
Isolation of cDNA fragments hybridizing to rat brain specific mRNAs
ESPOSITO, FRANCA;AMMENDOLA, ROSARIO;DUILIO, ANGELA;ZAMBRANO, NICOLA;RUSSO, TOMMASO;CIMINO, FILIBERTO
1990
Abstract
A cDNA minilibrary in pUC18 has been generated from 3-month-old rat brains. Two hundred clones were randomly selected and sequenced. Comparison with a nucleic acid and protein data bank revealed a number of cDNA fragments not homologous to any published sequence. Northern blot analyses of some of these clones yielded 5 brain-specific cDNA fragments. The full-length cDNA for one of these clones has been isolated and completely sequenced. It corresponds to an mRNA of about 1,500 nucleotides, which is present in brain and, to a lesser extent, in heart and skeletal muscle. Its expression is developmentally regulated in the rat brain from 14-day embryos to 3-month-old adults. A very recent comparison with the EMBL nucleotide sequence data bank showed that this mRNA codes for a brain-specific snRNP-associated protein.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
