We compared the sedimentation and FLOTAC techniques for the detection and quantification of Fasciola hepatica eggs in faecal samples obtained from 120 experimentally-infected rats before intervention, and in 42 rats after drug administration. Additionally, the average time for a single test was determined. A single FLOTAC showed a higher sensitivity (92.6%) than 2, 4 and 8 sedimentation readings (63.0-85.2%) for detecting F. hepatica eggs in rat faeces post-treatment. On average, it took 21. min to prepare and examine a single FLOTAC, whereas 114. min were needed for the sedimentation method including the reading of 8 slides. In both treated and untreated rats, the sedimentation method resulted in higher mean faecal egg counts (FECs) than FLOTAC (P<0.05). In view of the high sensitivity and efficiency, the FLOTAC technique holds promise for experimental work in the F. hepatica-rat model. Additional research is needed to determine the reasons for the observed differences in FECs.

Fasciola hepatica: Comparison of the sedimentation and FLOTAC techniques for the detection and quantification of faecal egg counts in rats / Duthaler, U.; Rinaldi, Laura; Maurelli, MARIA PAOLA; Vargas, M.; Utzinger, J.; Cringoli, Giuseppe; Keiser, J.. - In: EXPERIMENTAL PARASITOLOGY. - ISSN 0014-4894. - STAMPA. - 126:2(2010), pp. 161-166.

Fasciola hepatica: Comparison of the sedimentation and FLOTAC techniques for the detection and quantification of faecal egg counts in rats

RINALDI, LAURA;MAURELLI, MARIA PAOLA;CRINGOLI, GIUSEPPE;
2010

Abstract

We compared the sedimentation and FLOTAC techniques for the detection and quantification of Fasciola hepatica eggs in faecal samples obtained from 120 experimentally-infected rats before intervention, and in 42 rats after drug administration. Additionally, the average time for a single test was determined. A single FLOTAC showed a higher sensitivity (92.6%) than 2, 4 and 8 sedimentation readings (63.0-85.2%) for detecting F. hepatica eggs in rat faeces post-treatment. On average, it took 21. min to prepare and examine a single FLOTAC, whereas 114. min were needed for the sedimentation method including the reading of 8 slides. In both treated and untreated rats, the sedimentation method resulted in higher mean faecal egg counts (FECs) than FLOTAC (P<0.05). In view of the high sensitivity and efficiency, the FLOTAC technique holds promise for experimental work in the F. hepatica-rat model. Additional research is needed to determine the reasons for the observed differences in FECs.
2010
Fasciola hepatica: Comparison of the sedimentation and FLOTAC techniques for the detection and quantification of faecal egg counts in rats / Duthaler, U.; Rinaldi, Laura; Maurelli, MARIA PAOLA; Vargas, M.; Utzinger, J.; Cringoli, Giuseppe; Keiser, J.. - In: EXPERIMENTAL PARASITOLOGY. - ISSN 0014-4894. - STAMPA. - 126:2(2010), pp. 161-166.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11588/372517
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