p27BBP/eIF6 (β4 binding protein/eukaryotic initiation factor 6) is a highly conserved protein necessary for cell life. In adult eIF6 mice, a 50% decrease in the protein levels in all tissues is accompanied by a reduction in cell proliferation only in the liver, fat cells and cultured fibroblasts. During X. laevis embryogenesis expression of p27BBP/eIF6 is abundant in high proliferative territories. However, in Xenopus cell proliferation appears unaffected following p27BBP/eIF6 over-expression or down-regulation. Indeed, p27BBP/eIF6 is an anti-apoptotic factor acting upstream of Bcl2 that reduces endogenous apoptosis. We studied p27BBP/eIF6 protein localization in wild type embryos and compared it to proliferation and apoptosis. At the beginning of embryogenesis, high levels of p27BBP/eIF6, proliferation and apoptosis overlap. In later development stages high proliferation levels are present in the same regions where higher p27BBP/eIF6 expression is observed, while apoptosis does not appear specifically concentrated in the same sites. The higher presence of p27BBP/eIF6 would appear related to an increased need of apoptosis control in the regions where cell death is essential for normal development.

In X. laevis embryos high levels of the anti-apoptotic factor p27BBP/eif6 are stage-dependently found in BrdU and TUNEL- reactive territories / De Marco, N.; Campanella, C.; Carotenuto, Rosa. - In: ZYGOTE. - ISSN 0967-1994. - 19:2(2011), pp. 157-163. [10.1017/S0967199410000213]

In X. laevis embryos high levels of the anti-apoptotic factor p27BBP/eif6 are stage-dependently found in BrdU and TUNEL- reactive territories.

CAROTENUTO, ROSA
2011

Abstract

p27BBP/eIF6 (β4 binding protein/eukaryotic initiation factor 6) is a highly conserved protein necessary for cell life. In adult eIF6 mice, a 50% decrease in the protein levels in all tissues is accompanied by a reduction in cell proliferation only in the liver, fat cells and cultured fibroblasts. During X. laevis embryogenesis expression of p27BBP/eIF6 is abundant in high proliferative territories. However, in Xenopus cell proliferation appears unaffected following p27BBP/eIF6 over-expression or down-regulation. Indeed, p27BBP/eIF6 is an anti-apoptotic factor acting upstream of Bcl2 that reduces endogenous apoptosis. We studied p27BBP/eIF6 protein localization in wild type embryos and compared it to proliferation and apoptosis. At the beginning of embryogenesis, high levels of p27BBP/eIF6, proliferation and apoptosis overlap. In later development stages high proliferation levels are present in the same regions where higher p27BBP/eIF6 expression is observed, while apoptosis does not appear specifically concentrated in the same sites. The higher presence of p27BBP/eIF6 would appear related to an increased need of apoptosis control in the regions where cell death is essential for normal development.
2011
In X. laevis embryos high levels of the anti-apoptotic factor p27BBP/eif6 are stage-dependently found in BrdU and TUNEL- reactive territories / De Marco, N.; Campanella, C.; Carotenuto, Rosa. - In: ZYGOTE. - ISSN 0967-1994. - 19:2(2011), pp. 157-163. [10.1017/S0967199410000213]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11588/370846
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