INTRODUCTION: The Thyofanate Methyl is a pesticide and fungicide used in agriculture [1-2]. Our aim was therefore to assess the effects of toxicity and teratogenicity of acute doses of thiophanate methyl on embryonic development of Danio rerio[3]. METHODS: Embryos were exposed to different thyofanate metil concentrations, from 1µg/L to 10mg/L, for 144 hours at 28°C and were observed every 24 h for mortality and sublethal effects. The expression of MyoD, myogenin and myf5 genes was observed by in situ hybridization[4-5]. The 6 days larvae were labeled with anti-acetylated tubulin antibody, colored by diaminobenzidine (DAB) [6]. RESULTS and CONCLUSIONS: Survival and early development of the embryos were not obviously influenced by Thyofanate Methyl concentrations below 10mg/L, but at this concentration the embryos displayed alterations in neural development included weak tactile responses, loss of equilibrium and inability to swim. These treated embryos showed also: 1) an alteration of the spatial distribution of transcripts of MyoD and Myf-5 and myogenin signal was absent. 2) the localization of acetylated tubulin showed the breakage and the disorganization of the lateral line. Therefore, thiophanate-methyl at a concentration of 10 mg /L alters both skeletal muscle differentiaiton that the peripheral nervous system axogenesis. Our studies suggest that the negative effects of T.M. on vertebrate embryogenesis are substantial, therefore the T.M. is a possible contaminant of agriculture areas.

thiophanate methyl effects on Danio rerio embyonic development: morphological and molecular alterations / Chiarelli, Giovanni; M. P., Sommella; Lancieri, Massimo; Guida, Marco. - STAMPA. - (2010), pp. 278-279. (Intervento presentato al convegno 6th european conference on pesticides and related organic micropollutants in the enviroment and 12th symphosium on chemistry and fate of modern pesticides tenutosi a Matera nel september 5th-10th, 2010).

thiophanate methyl effects on Danio rerio embyonic development: morphological and molecular alterations

CHIARELLI, GIOVANNI;LANCIERI, MASSIMO;GUIDA, MARCO
2010

Abstract

INTRODUCTION: The Thyofanate Methyl is a pesticide and fungicide used in agriculture [1-2]. Our aim was therefore to assess the effects of toxicity and teratogenicity of acute doses of thiophanate methyl on embryonic development of Danio rerio[3]. METHODS: Embryos were exposed to different thyofanate metil concentrations, from 1µg/L to 10mg/L, for 144 hours at 28°C and were observed every 24 h for mortality and sublethal effects. The expression of MyoD, myogenin and myf5 genes was observed by in situ hybridization[4-5]. The 6 days larvae were labeled with anti-acetylated tubulin antibody, colored by diaminobenzidine (DAB) [6]. RESULTS and CONCLUSIONS: Survival and early development of the embryos were not obviously influenced by Thyofanate Methyl concentrations below 10mg/L, but at this concentration the embryos displayed alterations in neural development included weak tactile responses, loss of equilibrium and inability to swim. These treated embryos showed also: 1) an alteration of the spatial distribution of transcripts of MyoD and Myf-5 and myogenin signal was absent. 2) the localization of acetylated tubulin showed the breakage and the disorganization of the lateral line. Therefore, thiophanate-methyl at a concentration of 10 mg /L alters both skeletal muscle differentiaiton that the peripheral nervous system axogenesis. Our studies suggest that the negative effects of T.M. on vertebrate embryogenesis are substantial, therefore the T.M. is a possible contaminant of agriculture areas.
2010
9788875220983
thiophanate methyl effects on Danio rerio embyonic development: morphological and molecular alterations / Chiarelli, Giovanni; M. P., Sommella; Lancieri, Massimo; Guida, Marco. - STAMPA. - (2010), pp. 278-279. (Intervento presentato al convegno 6th european conference on pesticides and related organic micropollutants in the enviroment and 12th symphosium on chemistry and fate of modern pesticides tenutosi a Matera nel september 5th-10th, 2010).
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11588/370232
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