Reactive oxygen species, specifically hydrogen peroxide (H2O2), have a significant role in hormone production in thyroid tissue. Although recent studies have demonstrated that Dual oxidases (DUOX) are responsible for the H2O2 synthesis needed in thyroid hormone production, our data suggest a pivotal role for superoxide dismutase 3 (SOD3) as a major H2O2 producing enzyme. According to our results Sod3 is highly expressed in normal thyroid and becomes even more aboundant in rat goiter models. We showed TSH stimulated expression of Sod3 via PLC-Ca2+ and cAMP-PKA, a pathway that might be disrupted in thyroid cancer. In line with this finding, we demonstrated an oncogene dependent decrease in Sod3 mRNA expression synthesis in thyroid cancer cell models that corresponded to a similar decrease in clinical patient samples, suggesting that SOD3 could be used as a differentiation marker in thyroid cancer. Finally, the functional analysis in thyroid models indicated a moderate role for SOD3 in regulating normal thyroid cell proliferation being in line with our previous observations

Extracellular superoxide dismutase is a thyroid differentiation marker downregulated in cancer / Laatikainen, L.; Castellone, MARIA DOMENICA; Hebrant, A.; Hoste, C.; Cantisani, MARIA CARMELA; Laurila, J.; Salvatore, G.; Salerno, Paolo; Basolo, F.; Nasman, J.; Dumont, J. E.; Santoro, Massimo; Laukkanen, M.. - In: ENDOCRINE-RELATED CANCER. - ISSN 1351-0088. - STAMPA. - 17:3(2010), pp. 785-796. [10.1677/ERC-10-0021]

Extracellular superoxide dismutase is a thyroid differentiation marker downregulated in cancer.

CASTELLONE, MARIA DOMENICA;CANTISANI, MARIA CARMELA;SALERNO, PAOLO;SANTORO, MASSIMO;Laukkanen M.
2010

Abstract

Reactive oxygen species, specifically hydrogen peroxide (H2O2), have a significant role in hormone production in thyroid tissue. Although recent studies have demonstrated that Dual oxidases (DUOX) are responsible for the H2O2 synthesis needed in thyroid hormone production, our data suggest a pivotal role for superoxide dismutase 3 (SOD3) as a major H2O2 producing enzyme. According to our results Sod3 is highly expressed in normal thyroid and becomes even more aboundant in rat goiter models. We showed TSH stimulated expression of Sod3 via PLC-Ca2+ and cAMP-PKA, a pathway that might be disrupted in thyroid cancer. In line with this finding, we demonstrated an oncogene dependent decrease in Sod3 mRNA expression synthesis in thyroid cancer cell models that corresponded to a similar decrease in clinical patient samples, suggesting that SOD3 could be used as a differentiation marker in thyroid cancer. Finally, the functional analysis in thyroid models indicated a moderate role for SOD3 in regulating normal thyroid cell proliferation being in line with our previous observations
2010
Extracellular superoxide dismutase is a thyroid differentiation marker downregulated in cancer / Laatikainen, L.; Castellone, MARIA DOMENICA; Hebrant, A.; Hoste, C.; Cantisani, MARIA CARMELA; Laurila, J.; Salvatore, G.; Salerno, Paolo; Basolo, F.; Nasman, J.; Dumont, J. E.; Santoro, Massimo; Laukkanen, M.. - In: ENDOCRINE-RELATED CANCER. - ISSN 1351-0088. - STAMPA. - 17:3(2010), pp. 785-796. [10.1677/ERC-10-0021]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11588/369589
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