Many phytopathogenic bacteria display lipopolysaccharides (LPS) with the O-chain repeating unit [α-L-Rha-(1→3)-α-L-Rha-(1→3)-α-L-Rha-(1→2)]n. This trisaccharide unit was synthesized and oligomerized to obtain hexa- and nonasaccharides. The deprotected rhamnans were effective in suppressing the hypersensitive response (HR) and in inducing PR-1 gene expression in Arabidopsis thaliana. Conformational analysis of the oligorhamnans by NMR spectroscopy and molecular dynamics calculations revealed that a coiled structure develops with increasing chain length of the oligosaccharide. This is associated with increasing efficacy in HR suppression and PR-1 gene expression. We therefore infer that the coiled structure of phytopathogenic bacteria is a plant-recognizable pathogen-associated molecular pattern (PAMP).

Structure-dependent modulation of a pathogen response in plants by synthetic O-antigen polysaccharides / Bedini, Emiliano; DE CASTRO, Cristina; Mangoni, Lorenzo; Parrilli, Michelangelo; G., Erbs; J., MAXWELL DOW; MARI ANNE, Newman; C., Unverzagt. - In: JOURNAL OF THE AMERICAN CHEMICAL SOCIETY. - ISSN 0002-7863. - STAMPA. - 127:(2005), pp. 2414-2416. [10.1021/ja0452166]

Structure-dependent modulation of a pathogen response in plants by synthetic O-antigen polysaccharides

BEDINI, EMILIANO;DE CASTRO, CRISTINA;MANGONI, LORENZO;PARRILLI, MICHELANGELO;
2005

Abstract

Many phytopathogenic bacteria display lipopolysaccharides (LPS) with the O-chain repeating unit [α-L-Rha-(1→3)-α-L-Rha-(1→3)-α-L-Rha-(1→2)]n. This trisaccharide unit was synthesized and oligomerized to obtain hexa- and nonasaccharides. The deprotected rhamnans were effective in suppressing the hypersensitive response (HR) and in inducing PR-1 gene expression in Arabidopsis thaliana. Conformational analysis of the oligorhamnans by NMR spectroscopy and molecular dynamics calculations revealed that a coiled structure develops with increasing chain length of the oligosaccharide. This is associated with increasing efficacy in HR suppression and PR-1 gene expression. We therefore infer that the coiled structure of phytopathogenic bacteria is a plant-recognizable pathogen-associated molecular pattern (PAMP).
2005
Structure-dependent modulation of a pathogen response in plants by synthetic O-antigen polysaccharides / Bedini, Emiliano; DE CASTRO, Cristina; Mangoni, Lorenzo; Parrilli, Michelangelo; G., Erbs; J., MAXWELL DOW; MARI ANNE, Newman; C., Unverzagt. - In: JOURNAL OF THE AMERICAN CHEMICAL SOCIETY. - ISSN 0002-7863. - STAMPA. - 127:(2005), pp. 2414-2416. [10.1021/ja0452166]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11588/204344
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