A cross-sectional serological survey was conducted in order to evaluate, irrespective of abortion, the Toxoplasma gondii infection in pastured sheep from the Campania region of southern Italy. A geographical information system was used in order to uniformly sample the ovine farms (n = 117) throughout the entire region. Blood and milk samples were collected from 10 adult sheeps (>18 months) on each farm (total number = 1170 sheeps). Serum samples were tested for the presence of IgG antibodies to T. gondii using a commercial indirect fluorescent antibody test. For each farm, the 10 milk samples collected were pooled in order to obtain a single milk sample per farm (total number = 117 milk samples). The 77.8% (91/117) of the farms and the 28.5% (333/11,170) of the sheep resulted positive by serology. In addition, the presence of T. gondii DNA was detected by PCR in 4 milk samples out of the 117 examined (3.4%).
Toxoplasma gondii in sheep from the Campania region (Italy) / Fusco, G.; Rinaldi, Laura; Guarino, A.; Proroga, Y. T.; Pesce, A.; Giuseppina, D. M.; Cringoli, Giuseppe. - In: VETERINARY PARASITOLOGY. - ISSN 0304-4017. - STAMPA. - 149:3-4(2007), pp. 271-274.
Toxoplasma gondii in sheep from the Campania region (Italy)
RINALDI, LAURA;CRINGOLI, GIUSEPPE
2007
Abstract
A cross-sectional serological survey was conducted in order to evaluate, irrespective of abortion, the Toxoplasma gondii infection in pastured sheep from the Campania region of southern Italy. A geographical information system was used in order to uniformly sample the ovine farms (n = 117) throughout the entire region. Blood and milk samples were collected from 10 adult sheeps (>18 months) on each farm (total number = 1170 sheeps). Serum samples were tested for the presence of IgG antibodies to T. gondii using a commercial indirect fluorescent antibody test. For each farm, the 10 milk samples collected were pooled in order to obtain a single milk sample per farm (total number = 117 milk samples). The 77.8% (91/117) of the farms and the 28.5% (333/11,170) of the sheep resulted positive by serology. In addition, the presence of T. gondii DNA was detected by PCR in 4 milk samples out of the 117 examined (3.4%).File | Dimensione | Formato | |
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