A major objective in protein science is the design of enzymes with novel catalytic activities that are tailored to specific applications. Such enzymes may have great potential in biocatalysis and biosensor technology, such as in degradation of pollutants and biomass, and in drug and food processing. To reach this objective, investigations into the basic biochemical functioning of metalloproteins are still required. In this perspective, metalloprotein design provides a powerful approach first to contribute to a more comprehensive understanding of the way metalloproteins function in biology, with the ultimate goal of developing novel biocatalysts and sensing devices. Metalloprotein mimetics have been developed through the introduction of novel metal-binding sites into naturally occurring proteins as well as through de novo protein design. We have approached the challenge of reproducing metalloprotein active sites by using a miniaturization process. We centered our attention on iron-containing proteins, and we developed models for heme proteins and diironeoxo proteins. In this paper we summarize the results we obtained on the design, structural, and functional properties of DFs, a family of artificial diiron proteins.

Diiron-containing metalloproteins: Developing functional models / O., Maglio; Nastri, Flavia; R., Torres; M., Faiella; Pavone, Vincenzo; W. F., Degrado; Lombardi, Angelina. - In: COMPTES RENDUS CHIMIE. - ISSN 1631-0748. - STAMPA. - 10:(2007), pp. 703-720. [10.1016/j.crci.2007.03.010]

Diiron-containing metalloproteins: Developing functional models

NASTRI, FLAVIA;PAVONE, VINCENZO;LOMBARDI, ANGELINA
2007

Abstract

A major objective in protein science is the design of enzymes with novel catalytic activities that are tailored to specific applications. Such enzymes may have great potential in biocatalysis and biosensor technology, such as in degradation of pollutants and biomass, and in drug and food processing. To reach this objective, investigations into the basic biochemical functioning of metalloproteins are still required. In this perspective, metalloprotein design provides a powerful approach first to contribute to a more comprehensive understanding of the way metalloproteins function in biology, with the ultimate goal of developing novel biocatalysts and sensing devices. Metalloprotein mimetics have been developed through the introduction of novel metal-binding sites into naturally occurring proteins as well as through de novo protein design. We have approached the challenge of reproducing metalloprotein active sites by using a miniaturization process. We centered our attention on iron-containing proteins, and we developed models for heme proteins and diironeoxo proteins. In this paper we summarize the results we obtained on the design, structural, and functional properties of DFs, a family of artificial diiron proteins.
2007
Diiron-containing metalloproteins: Developing functional models / O., Maglio; Nastri, Flavia; R., Torres; M., Faiella; Pavone, Vincenzo; W. F., Degrado; Lombardi, Angelina. - In: COMPTES RENDUS CHIMIE. - ISSN 1631-0748. - STAMPA. - 10:(2007), pp. 703-720. [10.1016/j.crci.2007.03.010]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11588/202234
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