Isothermal titration calorimetry (ITC) is a sensitive technique for probing bimolecular processes and can provide direct information about the binding affinity and stoichiometry and the key thermodynamic parameters involved. ITC has been used to investigate the interaction of the ligand H2TMPyP to the two DNA quadruplexes, [d(AGGGT)]4 and [d(TGGGGT)]4. Analysis of the ITC data reveals that porphyrin/quadruplex binding stoichiometry under saturating conditions is 1:2 for [d(AGGGT)]4 and 2:1 for [d(TGGGGT)]4, respectively.
Interaction of Porphyrin with G-Quadruplex Structures / Erra, E; Petraccone, Luigi; Esposito, Veronica; Randazzo, Antonio; Mayol, Luciano; Ladbury, J; Barone, Guido; Giancola, Concetta. - In: NUCLEOSIDES, NUCLEOTIDES & NUCLEIC ACIDS. - ISSN 1525-7770. - ELETTRONICO. - 24:(2005), pp. 753-756. [10.1081/NCN-200060070]
Interaction of Porphyrin with G-Quadruplex Structures
PETRACCONE, LUIGI;ESPOSITO, VERONICA;RANDAZZO, ANTONIO;MAYOL, LUCIANO;BARONE, GUIDO;GIANCOLA, CONCETTA
2005
Abstract
Isothermal titration calorimetry (ITC) is a sensitive technique for probing bimolecular processes and can provide direct information about the binding affinity and stoichiometry and the key thermodynamic parameters involved. ITC has been used to investigate the interaction of the ligand H2TMPyP to the two DNA quadruplexes, [d(AGGGT)]4 and [d(TGGGGT)]4. Analysis of the ITC data reveals that porphyrin/quadruplex binding stoichiometry under saturating conditions is 1:2 for [d(AGGGT)]4 and 2:1 for [d(TGGGGT)]4, respectively.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
