Ochratoxin A (OTA) is a secondary fungal metabolite produced by Aspergillus and Penicillium strains that elicits a broad spectrum of toxicological effects in animals and man. A single oral OTA administration (10 mg/kg) in mice induced after 24 h oxidative damage and polymorphonuclear leukocyte (PMN) infiltration in parenchymal organs. In fact, OTA treatment increased lipid peroxidation (via malondialdehyde formation) in kidney and liver and PMN accumulation in duodenum, as shown by myeloperoxidase activity. Following in vivo OTA treatment an increase of cyclooxygenase-2 and of heat shock protein 72 expression was evidenced in peritoneal macrophage lysates by Western blot. That OTA modulates these proteins involved in the inflammatory process indicates that the mycotoxin is able to activate immune cells. This study suggests that the oxidative stress, the neutrophil accumulation in parenchymal tissues and the modulation of inflammatory parameters in peritoneal macrophages induced by OTA are involved in its toxicity, and represent early events related to several aspects of OTA mycotoxicosis.

Expression of COX-2 and hsp72 in peritoneal macrophages after an acute ochratoxin A treatment in mice / Ferrante, MARIA CARMELA; Bilancione, M.; MATTACE RASO, Giuseppina; Esposito, E.; Iacono, A.; Zaccaroni, A.; Meli, Rosaria. - In: LIFE SCIENCES. - ISSN 0024-3205. - STAMPA. - 79:13(2006), pp. 1242-1247. [10.1016/j.lfs.2006.03.031]

Expression of COX-2 and hsp72 in peritoneal macrophages after an acute ochratoxin A treatment in mice.

FERRANTE, MARIA CARMELA;MATTACE RASO, GIUSEPPINA;MELI, ROSARIA
2006

Abstract

Ochratoxin A (OTA) is a secondary fungal metabolite produced by Aspergillus and Penicillium strains that elicits a broad spectrum of toxicological effects in animals and man. A single oral OTA administration (10 mg/kg) in mice induced after 24 h oxidative damage and polymorphonuclear leukocyte (PMN) infiltration in parenchymal organs. In fact, OTA treatment increased lipid peroxidation (via malondialdehyde formation) in kidney and liver and PMN accumulation in duodenum, as shown by myeloperoxidase activity. Following in vivo OTA treatment an increase of cyclooxygenase-2 and of heat shock protein 72 expression was evidenced in peritoneal macrophage lysates by Western blot. That OTA modulates these proteins involved in the inflammatory process indicates that the mycotoxin is able to activate immune cells. This study suggests that the oxidative stress, the neutrophil accumulation in parenchymal tissues and the modulation of inflammatory parameters in peritoneal macrophages induced by OTA are involved in its toxicity, and represent early events related to several aspects of OTA mycotoxicosis.
2006
Expression of COX-2 and hsp72 in peritoneal macrophages after an acute ochratoxin A treatment in mice / Ferrante, MARIA CARMELA; Bilancione, M.; MATTACE RASO, Giuseppina; Esposito, E.; Iacono, A.; Zaccaroni, A.; Meli, Rosaria. - In: LIFE SCIENCES. - ISSN 0024-3205. - STAMPA. - 79:13(2006), pp. 1242-1247. [10.1016/j.lfs.2006.03.031]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11588/107306
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