Differential scanning calorimetry (DSC) and circular dichroism (CD) techniques were used to investigate the physico-chemical properties of the quadruplexes formed by the two different truncations of human telomeric sequence d(TAGGGT) and d(AGGGT), where the adenines were substituted by 2′-deoxy-8-(hydroxyl)adenosine (A → A OH ). CD spectra show that the modified sequences are able to form parallel-stranded quadruplex structure. Analysis of the thermodynamic parameters reveals that the introduction of the modified adenine affects in different way the thermal stability of the [d(TAGGGT)] 4 and [d(AGGGT)] 4 quadruplexes.
Effect of the incorporation of 2'-Deoxy-8-(hydroxyl)adenosine on the stability of quadruplexes formed by modified human telomeric DNA / Petraccone, Luigi; I., Duro; E., Erra; Randazzo, Antonio; A., Virno; Giancola, Concetta. - In: NUCLEOSIDES, NUCLEOTIDES & NUCLEIC ACIDS. - ISSN 1525-7770. - STAMPA. - 26:6-7(2007), pp. 675-679. [10.1080/15257770701490597]
Effect of the incorporation of 2'-Deoxy-8-(hydroxyl)adenosine on the stability of quadruplexes formed by modified human telomeric DNA
PETRACCONE, LUIGI;RANDAZZO, ANTONIO;GIANCOLA, CONCETTA
2007
Abstract
Differential scanning calorimetry (DSC) and circular dichroism (CD) techniques were used to investigate the physico-chemical properties of the quadruplexes formed by the two different truncations of human telomeric sequence d(TAGGGT) and d(AGGGT), where the adenines were substituted by 2′-deoxy-8-(hydroxyl)adenosine (A → A OH ). CD spectra show that the modified sequences are able to form parallel-stranded quadruplex structure. Analysis of the thermodynamic parameters reveals that the introduction of the modified adenine affects in different way the thermal stability of the [d(TAGGGT)] 4 and [d(AGGGT)] 4 quadruplexes.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
